Journal: Oncotarget

Article Title: Immunohistochemical profiling of receptor tyrosine kinases, MED12, and TGF-βRII of surgically resected small cell lung cancer, and the potential of c-kit as a prognostic marker

doi: 10.18632/oncotarget.14410

Figure Lengend Snippet: Distribution of RTKs, MED12, and TGF-βRII stratified by IHC total score in SCLC tumors as shown in Figure 2 (n=125)

Article Snippet: Rabbit anti-human polyclonal MED12 antibody (NB100-2357, Novus Biologicals, Littleton, CO, USA), rabbit anti-human polyclonal TGF-βRII antibody (NB100-91994, Novus Biologicals), rabbit anti-human polyclonal c-kit antibody (A4502, Dako, Glostrup, Denmark), rabbit anti-human polyclonal HER2 antibody (A0485, Dako), rabbit anti-human monoclonal c-Met antibody (EP1454Y, Abcam, Cambridge, UK), rabbit anti-human monoclonal VEGFRII antibody (#2479, CST, Danvers, MA, USA), mouse anti-human monoclonal EGFR antibody (31G7, Nichirei Biosciences, Tokyo, Japan), and mouse anti-human monoclonal ALK antibody (5A4, ab17127, Abcam) were used as first antibodies.

Techniques:

Journal: Oncotarget

Article Title: Immunohistochemical profiling of receptor tyrosine kinases, MED12, and TGF-βRII of surgically resected small cell lung cancer, and the potential of c-kit as a prognostic marker

doi: 10.18632/oncotarget.14410

Figure Lengend Snippet: Univariate analysis of the association between molecular expression and OS (n=107)

Article Snippet: Rabbit anti-human polyclonal MED12 antibody (NB100-2357, Novus Biologicals, Littleton, CO, USA), rabbit anti-human polyclonal TGF-βRII antibody (NB100-91994, Novus Biologicals), rabbit anti-human polyclonal c-kit antibody (A4502, Dako, Glostrup, Denmark), rabbit anti-human polyclonal HER2 antibody (A0485, Dako), rabbit anti-human monoclonal c-Met antibody (EP1454Y, Abcam, Cambridge, UK), rabbit anti-human monoclonal VEGFRII antibody (#2479, CST, Danvers, MA, USA), mouse anti-human monoclonal EGFR antibody (31G7, Nichirei Biosciences, Tokyo, Japan), and mouse anti-human monoclonal ALK antibody (5A4, ab17127, Abcam) were used as first antibodies.

Techniques: Expressing

Journal: Cell reports

Article Title: Modeling alcohol-associated liver disease in a human Liver-Chip

doi: 10.1016/j.celrep.2021.109393

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human Albumin SimpleStep ELISA® Kit , Abcam , ab179887.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Amplex Red Cholesterol Assay, Glucose Assay, Software

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Preconditioning of Human Decidua Basalis Mesenchymal Stem/Stromal Cells with Glucose Increased Their Engraftment and Anti-diabetic Properties

doi: 10.1007/s13770-020-00239-7

Figure Lengend Snippet: DBMSC proliferation groups. A Group 1 consisted of DBMSC cultured alone in a complete DBMSC culture medium. B Group 2 consisted of DBMSC cultured with different concentrations (25–400 mM) of glucose in a complete DBMSC culture medium. C Group 3 consisted of DBMSC pretreated with 200 mM glucose for 72 h [200 (pre)], harvested and then re-cultured alone in a complete DBMSC culture medium. D DBMSCs were seeded in a 16-well plate (E-Plate 16). The culture plates were then placed in the xCELLigence system at 37 °C in a cell culture incubator, and DBMSC cell index was then monitored. DBMSC proliferation in response to different glucose concentrations by the xCELLigence system. As compared to untreated DBMSCs, DBMSC proliferation was unchanged at 25 mM glucose ( p > 0.05) but significantly increased at 50 and 200 mM glucose and then significantly reduced at 400 mM glucose, after 24 h in culture. E At 48 h in culture, and as compared to untreated DBMSCs, DBMSC proliferation was unchanged at 50 mM glucose ( p > 0.05) but significantly increased at 200 mM glucose and then significantly reduced at 25 and 400 mM glucose. F At 72 h in culture, and as compared to untreated DBMSCs, DBMSC proliferation significantly increased at 200 mM glucose but was significantly reduced at 25, 50 and 400 mM glucose. G - I The reversibility of DBMSC proliferation in response to glucose. DBMSCs were initially cultured with 200 mM glucose for 72 h and their proliferation was then determined using the xCELLigence system. At 24–72 h in culture, and as compared to untreated DBMSCs and DBMSC-treated with 200 mM glucose [200 (I)], the proliferation of DBMSC pretreated with 200 mM glucose [200 (pre)] significantly reduced

Article Snippet: DBMSCs were cultured in a complete DBMSC culture medium [DMEM-F12 medium containing 10% MSCFBS (Mesenchymal Stem Cell certified fetal bovine serum, catalogue number 12-662-011, Life Technologies, Grand Island, NY, USA), and antibiotics (100 μg/mL streptomycin and 100 U/mL penicillin)], while HUVEC were cultured in a complete endothelial cell growth medium (Catalogue number PCS-100-041TM, ATCC, Manassas, VA, USA).

Techniques: Cell Culture

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Preconditioning of Human Decidua Basalis Mesenchymal Stem/Stromal Cells with Glucose Increased Their Engraftment and Anti-diabetic Properties

doi: 10.1007/s13770-020-00239-7

Figure Lengend Snippet: DBMSC migration groups. A Group 1 consisted of DBMSCs cultured alone in the upper chamber. B Group 2 consisted of DBMSCs cultured alone in the upper chamber while 200 mM glucose was added to the lower chamber. C Group 3 consisted of DBMSCs pre-treated with 200 mM glucose for 72 h (200(pre)) harvetsed and then re-cultured alone in the upper chamber while 200 mM glucose was added to the lower chamber. D DBMSCs were seeded in DBMSC serum free medium in the upper chamber of the CIM migration plate while DBMSC culture medium containing 30% FBS was added to the lower chambers. At 24 h, DBMSC [DB (T 200)] migration in response to 200 mM glucose significantly increased as compared to untreated DBMSCs (DB). The migration of DBMSCs pretreated with 200 mM glucose for 72 h (Pre-DB) in response to 200 mM glucose [Pre-DB (To 200)] significantly increased as compared to untreated DBMSCs (DB), but was unchanged as compared to DBMSC migrating in response to 200 mM glucose [DB (To 200)], p > 0.05. E The effect of glucose on DBMSC invasion through endothelial cells by the xCELLigence system. At 10 h, the pretreatment with 200 mM glucose for 72 h [200 (Pre)] significantly increased DBMSC invasion as compared to untreated DBMSCs and DBMSC cultured with 200 mM glucose while the addition of 200 mM glucose [200 (in)] during the invasion experiment had no significant effect on DBMSC invasion as compared to untreated DBMSCs. Each experiment was performed in triplicate and repeated with five independent DBMSC (passage 3) preparations. * p < 0.05. Bars represent standard errors

Article Snippet: DBMSCs were cultured in a complete DBMSC culture medium [DMEM-F12 medium containing 10% MSCFBS (Mesenchymal Stem Cell certified fetal bovine serum, catalogue number 12-662-011, Life Technologies, Grand Island, NY, USA), and antibiotics (100 μg/mL streptomycin and 100 U/mL penicillin)], while HUVEC were cultured in a complete endothelial cell growth medium (Catalogue number PCS-100-041TM, ATCC, Manassas, VA, USA).

Techniques: Migration, Cell Culture

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Preconditioning of Human Decidua Basalis Mesenchymal Stem/Stromal Cells with Glucose Increased Their Engraftment and Anti-diabetic Properties

doi: 10.1007/s13770-020-00239-7

Figure Lengend Snippet: BMSCs were cultured with 200 mM glucose [200 (I)] and their adhesion was then determined using the xCELLigence system. As compared to untreated DBMSCs, the treatment with 200 mM glucose had no significant effect on DBMSC adhesion at 2 h in culture ( p > 0.05) while the adhesion of DBMSC pretreated with 200 mM glucose for 72 h was significantly increased at 2 h as compared to untreated DBMSCs, and DBMSC cultured with 200 mM glucose [200 (I)]. Each experiment was performed in triplicate and repeated with five independent DBMSC (passage 3) preparations. * p < 0.05. Bars represent standard errors

Article Snippet: DBMSCs were cultured in a complete DBMSC culture medium [DMEM-F12 medium containing 10% MSCFBS (Mesenchymal Stem Cell certified fetal bovine serum, catalogue number 12-662-011, Life Technologies, Grand Island, NY, USA), and antibiotics (100 μg/mL streptomycin and 100 U/mL penicillin)], while HUVEC were cultured in a complete endothelial cell growth medium (Catalogue number PCS-100-041TM, ATCC, Manassas, VA, USA).

Techniques: Cell Culture

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Preconditioning of Human Decidua Basalis Mesenchymal Stem/Stromal Cells with Glucose Increased Their Engraftment and Anti-diabetic Properties

doi: 10.1007/s13770-020-00239-7

Figure Lengend Snippet: Flow cytometric analysis of DBMSC expression of immune markers. A - C The treatment with 200 mM glucose significantly increased the DBMSCs [200 (pre)] expression of ICAM-1, had no significant effect on IL-12 expression, p > 0.05, and significantly increased the expression of B7H4, as compared untreated DBMSCs. Each experiment was performed in triplicate and repeated with five independent DBMSC (passage 3) preparations. * p < 0.05. Bars represent standard errors

Article Snippet: DBMSCs were cultured in a complete DBMSC culture medium [DMEM-F12 medium containing 10% MSCFBS (Mesenchymal Stem Cell certified fetal bovine serum, catalogue number 12-662-011, Life Technologies, Grand Island, NY, USA), and antibiotics (100 μg/mL streptomycin and 100 U/mL penicillin)], while HUVEC were cultured in a complete endothelial cell growth medium (Catalogue number PCS-100-041TM, ATCC, Manassas, VA, USA).

Techniques: Expressing

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Preconditioning of Human Decidua Basalis Mesenchymal Stem/Stromal Cells with Glucose Increased Their Engraftment and Anti-diabetic Properties

doi: 10.1007/s13770-020-00239-7

Figure Lengend Snippet: Glucose effects on DBMSC expression of oxidative genes with survival, anti-apoptotic, proliferation, and migration properties. DBMSCs were untreated (DBMSC) or treated with 200 mM glucose (TDBMSC) for 72 h

Article Snippet: DBMSCs were cultured in a complete DBMSC culture medium [DMEM-F12 medium containing 10% MSCFBS (Mesenchymal Stem Cell certified fetal bovine serum, catalogue number 12-662-011, Life Technologies, Grand Island, NY, USA), and antibiotics (100 μg/mL streptomycin and 100 U/mL penicillin)], while HUVEC were cultured in a complete endothelial cell growth medium (Catalogue number PCS-100-041TM, ATCC, Manassas, VA, USA).

Techniques: Expressing, Migration

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Preconditioning of Human Decidua Basalis Mesenchymal Stem/Stromal Cells with Glucose Increased Their Engraftment and Anti-diabetic Properties

doi: 10.1007/s13770-020-00239-7

Figure Lengend Snippet: Glucose effects on DBMSC expression of oxidative genes with pro-oxidant and antioxidant properties. DBMSCs were untreated (DBMSC) or treated with 200 mM glucose (TDBMSC) for 72 h

Article Snippet: DBMSCs were cultured in a complete DBMSC culture medium [DMEM-F12 medium containing 10% MSCFBS (Mesenchymal Stem Cell certified fetal bovine serum, catalogue number 12-662-011, Life Technologies, Grand Island, NY, USA), and antibiotics (100 μg/mL streptomycin and 100 U/mL penicillin)], while HUVEC were cultured in a complete endothelial cell growth medium (Catalogue number PCS-100-041TM, ATCC, Manassas, VA, USA).

Techniques: Expressing

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Preconditioning of Human Decidua Basalis Mesenchymal Stem/Stromal Cells with Glucose Increased Their Engraftment and Anti-diabetic Properties

doi: 10.1007/s13770-020-00239-7

Figure Lengend Snippet: Glucose increased DBMSC expression of genes with antioxidant, anti-inflammatory, anti-chemoattractant, and antimicrobial properties. DBMSCs were untreated (DBMSC) or treated with 200 mM glucose (TDBMSC) for 72 h

Article Snippet: DBMSCs were cultured in a complete DBMSC culture medium [DMEM-F12 medium containing 10% MSCFBS (Mesenchymal Stem Cell certified fetal bovine serum, catalogue number 12-662-011, Life Technologies, Grand Island, NY, USA), and antibiotics (100 μg/mL streptomycin and 100 U/mL penicillin)], while HUVEC were cultured in a complete endothelial cell growth medium (Catalogue number PCS-100-041TM, ATCC, Manassas, VA, USA).

Techniques: Expressing, Clinical Proteomics

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Preconditioning of Human Decidua Basalis Mesenchymal Stem/Stromal Cells with Glucose Increased Their Engraftment and Anti-diabetic Properties

doi: 10.1007/s13770-020-00239-7

Figure Lengend Snippet: Glucose effects on DBMSC expression of oxidative genes. DBMSCs were untreated (DBMSC) or treated with 200 mM glucose (TDBMSC) for 72 h

Article Snippet: DBMSCs were cultured in a complete DBMSC culture medium [DMEM-F12 medium containing 10% MSCFBS (Mesenchymal Stem Cell certified fetal bovine serum, catalogue number 12-662-011, Life Technologies, Grand Island, NY, USA), and antibiotics (100 μg/mL streptomycin and 100 U/mL penicillin)], while HUVEC were cultured in a complete endothelial cell growth medium (Catalogue number PCS-100-041TM, ATCC, Manassas, VA, USA).

Techniques: Expressing

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Preconditioning of Human Decidua Basalis Mesenchymal Stem/Stromal Cells with Glucose Increased Their Engraftment and Anti-diabetic Properties

doi: 10.1007/s13770-020-00239-7

Figure Lengend Snippet: Glucose effects on DBMSC expression of oxidative genes. DBMSCs were untreated (DBMSC) or treated with 200 mM glucose (TDBMSC) for 72 h

Article Snippet: DBMSCs were cultured in a complete DBMSC culture medium [DMEM-F12 medium containing 10% MSCFBS (Mesenchymal Stem Cell certified fetal bovine serum, catalogue number 12-662-011, Life Technologies, Grand Island, NY, USA), and antibiotics (100 μg/mL streptomycin and 100 U/mL penicillin)], while HUVEC were cultured in a complete endothelial cell growth medium (Catalogue number PCS-100-041TM, ATCC, Manassas, VA, USA).

Techniques: Expressing, Membrane